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KMID : 0377519770020010089
Chung-Ang Journal of Medicine
1977 Volume.2 No. 1 p.89 ~ p.92
An Autoradiographic Study on the Distribution of ^14C-Glycine in Clonorchis sinesis
Lee Soon-Hyung

Song Chul-Yong
Abstract
In order to know an aspect of protein metabolism in chinese liverfluke, Clonorchis sinensis, an autoradiographic study was performed. A batch of 25ml Erlenmeyer flasks, each flask containing 10 worms of C. sinensis and 10ml of Tyrode medium with 2.5¥ì Ci/ml of ^14C-glycine, was incubated for 1 hour in Dubnoff metabolic shaking incubator at 37¡É. Those worms were processed for microautoradiography immediately after the incubation, and following results were obtained from the autoradiographs. 1. The densities of black silver grains derived from ^14C-glycine were the most apparent in the subparenchymal cells, intestinal epithelium, vitelline gland cells, ovary and the wall of the seminal vesicle. 2. Moderate grade of densities were observed in the tegument, oral sucker, pharynx, intestinal content and in the testes. 3. The reticular tissue, ventral sucker, uterus with eggs, Seminal receptacle and the content of seminal vesicle showed trace amount of silver grains.
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